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a Exp. ii Promoter sequence ..CAATTGCTGGCA... ...CGCGAATTATCA... viii *.. ...TTCAGATGCATC... i i i i i i i i i : : : : : : : : : : : : :1 : 11 : : 1 1 1 : 1 1 : 1 1 : : : i : i : : : : : : : : : : : ÁTGCAT.. 1 1 : : : 1 : : ! i i : 1 i 1 i i i vii iv : : : : :: : : : : : : : : ! : : ! ..TACTATACCGAA... vi Participants develop Evaluate models using 6,739,258 sequence- sequence-to-expression designed sequences expression pairs models with biological insight b 17 C 18 16 76 15 14 15 13 12 11 10 9 8 7 6 5 4 32 321 1 BHI mt BHI mt NAD wztr NAD BUGF wztr BUGF BioNML BioNML Unlock_DNA Camformers Unlock_DNA Camformers Autosome.org Autosome.org High schoolers High schoolers e Performance in Pearson's r2 Performance in Spearman's p Autosome.org 0.82 0.950.51 0.51 0.78 0.96 0.94 0.73 0.96 0.91 Autosome.org b.85 0.980.680.640.880.98 0.97 0.71 0.97 0.94 BHI 0.79 0.930.43 0.44 0.74 0.95 0.91 0.7 0.96 0.9 BHI 0.84 0.97 0.680.630.86 0.98 0.95 0.7 0.97 0.94 Unlock_DNA 0.77 0.920.38 0.39 0.7 0.94 0.88 0.68 0.94 0.89 Unlock_ DNA 0.820.96 0.58 0.6 0.840.97 0.93 0.68 0.96 0.93 Camformers 0.75 0.91 0.31 0.39 0.68 0.94 0.89 0.67 0.95 0.81 Camformers 0.82 0.96 0.57 0.61 0.82 0.970.940.67 0.96 0.91 NAD 0.75 0.91 0.27 0.32 0.66 0.94 0.86 0.67 0.95 0.88 NAD 0.820.95 0.58 0.59 0.81 0.97 0.920.68 0.96 0.92 p wztr 0.76 0.920.27 0.3 0.68 0.95 0.91 0.68 0.940.89 wztr 0.81 0.96 0.61 0.3 0.810.98 0.94 0.68 0.96 0.93 High schoolers 0.75 0.91 0.35 0.32 0.66 0.94 0.86 0.67 0.940.87 High schoolers 0.81 0.960.53 0.5 0.81 0.97 0.91 0.67 0.96 0.92 BioNML 0.75 0.9 0.38 0.4 0.670.93 0.85 0.65 0.920.87 BioNML 0.8 0.95 0.520.590.810.97 0.9 0.66 0.94 0.92 BUGF 0.74 0.9 0.34 0.4 0.68 0.93 0.87 0.64 0.940.83 BUGF 0.81 0.95 0.540.59 0.820.96 0.92 0.66 0.95 0.9 mt 0.74 0.9 0.36 0.37 0.68 0.93 0.86 0.65 0.930.84 mt 0.8 0.95 0.45 0.59 0.82 0.97 0.92 0.65 0.95 0.89 Reference model 0.7 0.88 0.26 0.27 0.64 0.91 0.77 0.62 0.9 0.75 Reference model 0.76 0.94 0.31 0.42 0.8 0.96 0.86 0.64 0.93 0.87 High Low SNVs High Low Native Native SNVs Random Random Challenging Motif tiling Challenging Motif tiling Pearson score All sequences All sequences Spearman score Motif perturbation Motif perturbation Test subsets Test subsets f 50 g 50 % 40 d % 40 30 30 20 20 10 10 0 0 High Low SNVs High Low Native Native SNVs Random Random Motif tiling Challenging Motif tiling Challenging Pearson score All sequences All sequences Spearman score Motif perturbation Motif perturbation Test subsets Test subsets